8KGS
Structure of African swine fever virus topoisomerase II in complex with dsDNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2021-10-12 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 85.077, 85.077, 210.180 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.780 - 2.603 |
R-factor | 0.2023 |
Rwork | 0.200 |
R-free | 0.24410 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.060 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.203 | 1.019 |
Rpim | 0.339 | |
Number of reflections | 14604 | 708 |
<I/σ(I)> | 11 | 1.38 |
Completeness [%] | 100.0 | 99.3 |
Redundancy | 19 | 9 |
CC(1/2) | 0.996 | 0.760 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 21% PEG 3350, 100 mM Hepes (pH 7.2) and 200 mM MgCl2 |