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8KGS

Structure of African swine fever virus topoisomerase II in complex with dsDNA

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU MICROMAX-007 HF
Temperature [K]100
Detector technologyIMAGE PLATE
Collection date2021-10-12
DetectorRIGAKU RAXIS IV++
Wavelength(s)1.5418
Spacegroup nameP 61 2 2
Unit cell lengths85.077, 85.077, 210.180
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution42.780 - 2.603
R-factor0.2023
Rwork0.200
R-free0.24410
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.010
RMSD bond angle1.060
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwarePHASER
Refinement softwarePHENIX ((1.12_2829: ???))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.640
High resolution limit [Å]2.6002.600
Rmerge0.2031.019
Rpim0.339
Number of reflections14604708
<I/σ(I)>111.38
Completeness [%]100.099.3
Redundancy199
CC(1/2)0.9960.760
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP293.1521% PEG 3350, 100 mM Hepes (pH 7.2) and 200 mM MgCl2

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PDB entries from 2024-11-13

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