8KG4

Crystal Structure of M- and C-Domains of the shaft pilin LrpA from Ligilactobacillus ruminis - orthorhombic form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98030
Spacegroup name	P 21 21 21
Unit cell lengths	41.825, 50.853, 125.488
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.710 - 1.200
R-factor	0.16956
Rwork	0.169
R-free	0.18761
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.657
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	REFMAC (5.8.0415)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.710	1.223
High resolution limit [Å]	1.200	1.200
Rmerge	0.096	0.779
Rmeas	0.102	0.834
Rpim	0.034	0.292
Number of reflections	84143	4207
<I/σ(I)>	12.8	4.6
Completeness [%]	99.6	94.6
Redundancy	8.6	7.7
CC(1/2)	0.996	0.888

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	295	0.1 M sodium acetate pH 4.6, 25% PEG 4000