8KG4
Crystal Structure of M- and C-Domains of the shaft pilin LrpA from Ligilactobacillus ruminis - orthorhombic form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98030 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.825, 50.853, 125.488 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.710 - 1.200 |
| R-factor | 0.16956 |
| Rwork | 0.169 |
| R-free | 0.18761 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.657 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.710 | 1.223 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.096 | 0.779 |
| Rmeas | 0.102 | 0.834 |
| Rpim | 0.034 | 0.292 |
| Number of reflections | 84143 | 4207 |
| <I/σ(I)> | 12.8 | 4.6 |
| Completeness [%] | 99.6 | 94.6 |
| Redundancy | 8.6 | 7.7 |
| CC(1/2) | 0.996 | 0.888 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 0.1 M sodium acetate pH 4.6, 25% PEG 4000 |
