8KG3
Structure of THOUSAND-GRAIN WEIGHT 6 (TGW6)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-19 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.999994 |
| Spacegroup name | P 1 |
| Unit cell lengths | 105.982, 108.520, 123.071 |
| Unit cell angles | 94.14, 98.74, 97.23 |
Refinement procedure
| Resolution | 48.990 - 2.600 |
| R-factor | 0.22716 |
| Rwork | 0.225 |
| R-free | 0.26025 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.202 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.720 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.096 | 0.736 |
| Number of reflections | 161474 | 8003 |
| <I/σ(I)> | 8.4 | 1.4 |
| Completeness [%] | 98.3 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.6 | 283 | 0.8M sodium citrate tribasic, 0.1M CHES-NaOH, pH 9.6, 3% (w/v) sucrose |
