8KEU
Crystal Structures of Endo-beta-1,4-xylanase II mutant T2CT28C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2022-11-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.119, 59.298, 69.782 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.060 - 1.100 |
| R-factor | 0.136 |
| Rwork | 0.135 |
| R-free | 0.14930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.022 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.060 | 1.139 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Number of reflections | 78472 | 6874 |
| <I/σ(I)> | 23.857 | 2 |
| Completeness [%] | 98.1 | 87.11 |
| Redundancy | 11.7 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 20% PEG 8000, 0.2M NaI, 0.1M MES |
