8KE8
Crystal structure of TetR-type transcriptional factor NalC from P. aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-06-14 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.00003 |
Spacegroup name | P 61 |
Unit cell lengths | 57.517, 57.517, 224.345 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.670 - 2.050 |
R-factor | 0.2129 |
Rwork | 0.210 |
R-free | 0.24920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | AlphaFold |
RMSD bond length | 0.002 |
RMSD bond angle | 0.531 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.19.1_4122: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.090 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.061 | 0.361 |
Number of reflections | 26116 | 1257 |
<I/σ(I)> | 34.52 | |
Completeness [%] | 99.8 | |
Redundancy | 11.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.25 | 293 | 0.2 M Ammonium chloride, 0.1 M MES pH 6.25, 1.0 M Potassium sodium tartrate |