8KD8

N(5)-hydroxyornithine:cis-anhydromevalonyl coenzyme A-N(5)-transacylase sidF N-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	62.200, 110.670, 69.474
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.438 - 2.581
Rwork	0.173
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	2.192
Data reduction software	XDS
Data scaling software	Aimless (0.7.9)
Phasing software	MrBUMP (2.2.6)
Refinement software	REFMAC (5.8.0415)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.438	2.700
High resolution limit [Å]	2.580	2.580
Number of reflections	7832	927
<I/σ(I)>	4.4	4.4
Completeness [%]	99.8	99.2
Redundancy	12.2	12.3
CC(1/2)	0.998	0.942

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		293	0.2M potassium sodium tartrate tetrahydrate, 20% w/v polyethylene glycol 3350, cryoprotected with 40% Morpheus precipitant mix 3