8KCL
Crystal Structure of M- and C-Domains of the shaft pilin LrpA from Ligilactobacillus ruminis - Triclinic form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98030 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.334, 47.677, 51.720 |
| Unit cell angles | 107.25, 101.52, 111.78 |
Refinement procedure
| Resolution | 39.460 - 1.470 |
| R-factor | 0.17545 |
| Rwork | 0.175 |
| R-free | 0.19106 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.460 | 1.491 |
| High resolution limit [Å] | 1.465 | 1.465 |
| Rmerge | 0.110 | 0.444 |
| Rmeas | 0.130 | 0.545 |
| Rpim | 0.069 | 0.308 |
| Number of reflections | 47229 | 2307 |
| <I/σ(I)> | 6.9 | 2.1 |
| Completeness [%] | 95.2 | 92.4 |
| Redundancy | 3.4 | 3 |
| CC(1/2) | 0.987 | 0.792 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2 M Lithium Sulphate, 0.1 M Tris pH 8.5, 30% PEG 4000 |
