8KCA

Crystal structure of DDX53 helicase domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97942
Spacegroup name	P 1 21 1
Unit cell lengths	41.866, 106.494, 41.943
Unit cell angles	90.00, 98.61, 90.00

Refinement procedure

Resolution	27.320 - 1.970
R-factor	0.189
Rwork	0.185
R-free	0.23140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.127
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.040
High resolution limit [Å]	1.970	1.970
Rmerge	0.082	0.640
Number of reflections	24420	2193
<I/σ(I)>	14.3
Completeness [%]	96.4
Redundancy	3.3
CC(1/2)	0.996	0.662

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	293	1 M trisodium citrate