8KBW

The crystal structure of syn-copalyl diphosphate synthase from Oryza sativa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 21 21 21
Unit cell lengths	129.702, 174.342, 296.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	97.620 - 3.490
R-factor	0.2027
Rwork	0.201
R-free	0.26270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.400
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	97.620	3.610
High resolution limit [Å]	3.490	3.490
Rpim	0.111
Number of reflections	85822	8453
<I/σ(I)>	10.14
Completeness [%]	96.5
Redundancy	13.5
CC(1/2)	0.995	0.511

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	2% v/v 1,4-dioxane, 0.1 M Tris pH 8.0, 15% w/v polyethylene glycol 3,350