8KBM
Structure of AcrIIA7 complexed with cGG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 88.065, 89.846, 63.214 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.770 - 1.380 |
| R-factor | 0.2035 |
| Rwork | 0.203 |
| R-free | 0.21810 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.398 |
| Data reduction software | xia2 |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.770 | 1.450 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Rmeas | 0.109 | 1.718 |
| Number of reflections | 97761 | 4952 |
| <I/σ(I)> | 15 | 2 |
| Completeness [%] | 97.9 | |
| Redundancy | 26.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 1.0 M Lithium chloride, 20% w/v Polyethylene glycol 6000 |
