8KB9
Structure of the WDR91 WD40 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-03-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 49.721, 154.353, 92.314 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.330 - 1.900 |
R-factor | 0.2136 |
Rwork | 0.212 |
R-free | 0.23840 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.545 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.330 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.055 | 1.081 |
Number of reflections | 28408 | 1807 |
<I/σ(I)> | 21.6 | |
Completeness [%] | 99.7 | |
Redundancy | 13.4 | |
CC(1/2) | 0.999 | 0.794 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1M ammonium citrate tribasic pH 7.0, 8% PEG 3350 |