8KB7

Crystal structure of UDP/mannose-bound AGO61/beta-1,4-N-Acetylglucosaminyltransferase 2 (POMGNT2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9000
Spacegroup name	P 21 21 21
Unit cell lengths	145.019, 150.002, 191.481
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.920 - 2.800
R-factor	0.19304
Rwork	0.191
R-free	0.22823
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.634
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AutoSol
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.970
High resolution limit [Å]	2.800	2.800
Rmerge	0.062	0.918
Number of reflections	97911	31767
<I/σ(I)>	13.5	1.2
Completeness [%]	99.7	99.3
Redundancy	3.6	3.4
CC(1/2)	0.999	0.569

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	12% PEG4000, 0.1 M sodium citrate tribasic (pH 6.0), and 0.1 M sodium chloride