8KAP

Glycoside hydrolase family 1 beta-glucosidase from Streptomyces griseus (ligand-free)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-07
Detector	DECTRIS PILATUS3 S 2M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	96.858, 97.756, 184.874
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.261 - 2.200
Rwork	0.232
R-free	0.25680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.863
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0415)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.430	2.240
High resolution limit [Å]	2.200	2.200
Number of reflections	89749	4548
<I/σ(I)>	8.8
Completeness [%]	100.0	100
Redundancy	6.6
CC(1/2)	0.991	0.685

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Bis-Tris (pH 5.5), 0.2 M ammonium acetate, 15% (w/v) PEG3350