8KAO
Glutamate dehydrogenase-69O
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.782, 153.599, 110.550 |
| Unit cell angles | 90.00, 115.58, 90.00 |
Refinement procedure
| Resolution | 45.040 - 2.550 |
| R-factor | 0.21054 |
| Rwork | 0.208 |
| R-free | 0.26022 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.321 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.040 | 2.630 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.166 | 1.164 |
| Rmeas | 0.180 | 1.259 |
| Rpim | 0.068 | 0.476 |
| Total number of observations | 30810 | |
| Number of reflections | 48864 | 4486 |
| <I/σ(I)> | 10.3 | 1.8 |
| Completeness [%] | 100.0 | |
| Redundancy | 6.9 | 6.9 |
| CC(1/2) | 0.995 | 0.708 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM Potassium phosphate monobasic/Sodium phosphate dibasic buffer (pH 6.2), 10% (w/v) polyethylene glycol (PEG) 3,000, and 10% (v/v) glycerol. |
