8KAK
Crystal structure of SpyCas9 in complex with sgRNA and 18nt target DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97852 |
| Spacegroup name | P 1 |
| Unit cell lengths | 70.742, 104.970, 184.309 |
| Unit cell angles | 75.82, 78.67, 72.63 |
Refinement procedure
| Resolution | 24.970 - 3.600 |
| R-factor | 0.2665 |
| Rwork | 0.265 |
| R-free | 0.29710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.595 |
| Data reduction software | HKL-3000 (7.21) |
| Data scaling software | HKL-3000 (7.21) |
| Phasing software | PHENIX (1.17.1_3660) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.720 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rpim | 0.176 | 0.708 |
| Number of reflections | 52766 | 2595 |
| <I/σ(I)> | 4.11 | |
| Completeness [%] | 94.6 | 93.2 |
| Redundancy | 2.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.15 M Ammonium sulfate, 0.1 M MES pH 6.0, 14.75% PEG4000 |
