8KAJ
Crystal structure of SpyCas9-crRNA-tracrRNA complex bound to 16nt target DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 144.735, 131.105, 146.631 |
| Unit cell angles | 90.00, 103.68, 90.00 |
Refinement procedure
| Resolution | 48.030 - 3.420 |
| R-factor | 0.2373 |
| Rwork | 0.237 |
| R-free | 0.24650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.551 |
| Data reduction software | HKL-3000 (7.21) |
| Data scaling software | HKL-3000 (7.21) |
| Phasing software | PHENIX (1.17.1_360) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.440 |
| High resolution limit [Å] | 3.380 | 3.380 |
| Rpim | 0.090 | 0.493 |
| Number of reflections | 97863 | 3645 |
| <I/σ(I)> | 8.71 | |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 6.1 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 1.0 M Sodium chloride, 0.1 M Sodium citrate pH 6.0, 12.75% PEG4000 |
