8KA5

Arabidopsis AP endonuclease ARP complex with 20bp THF-containing DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	P 32 2 1
Unit cell lengths	72.093, 72.093, 188.189
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.630 - 2.800
R-factor	0.2043
Rwork	0.201
R-free	0.25860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.216
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.120	1.933
Rmeas	0.124	1.984
Rpim	0.028	0.444
Number of reflections	14020	2079
<I/σ(I)>	18.8	2
Completeness [%]	95.9	99.7
Redundancy	19.3	19.4
CC(1/2)	0.999	0.833

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Ammonium acetate, 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 30% w/v Polyethylene glycol 4,000