8KA4

Arabidopsis AP endonuclease ARP complex with 21bp THF-containing DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	68.154, 102.215, 169.198
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.990 - 2.300
R-factor	0.1859
Rwork	0.183
R-free	0.23570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.989
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.128	0.786
Rmeas	0.139	0.853
Rpim	0.053	0.327
Number of reflections	49885	7544
<I/σ(I)>	11	2.3
Completeness [%]	93.6	98.1
Redundancy	6.7	6.7
CC(1/2)	0.997	0.771

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Ammonium acetate, 0.1 M HEPES pH 7.5, 25% w/v Polyethylene glycol 3,350