8KA3

Arabidopsis AP endonuclease ARP complex with 22bp THF-containing DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	C 1 2 1
Unit cell lengths	136.731, 52.295, 71.095
Unit cell angles	90.00, 117.03, 90.00

Refinement procedure

Resolution	29.700 - 3.000
R-factor	0.2447
Rwork	0.242
R-free	0.30200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.533
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.160
High resolution limit [Å]	3.000	3.000
Rmerge	0.137	1.182
Rmeas	0.149	1.283
Rpim	0.058	0.494
Number of reflections	8713	1348
<I/σ(I)>	10.3	1.7
Completeness [%]	94.8
Redundancy	6.5
CC(1/2)	0.998	0.678

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	25% (w/v) PEG 1500, 0.1M PCB/Sodium hydroxide pH 7.0