8KA2
Crystal structure of the RID-dependent transforming NADase domain (RDTND)/calmodulin-binding domain of Rho inactivation domain (RID-CBD) from Vibrio vulnificus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-07-06 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 6 |
Unit cell lengths | 207.726, 207.726, 54.647 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.420 - 3.380 |
R-factor | 0.28789 |
Rwork | 0.286 |
R-free | 0.32212 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.312 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.440 |
High resolution limit [Å] | 3.380 | 3.380 |
Number of reflections | 17817 | 721 |
<I/σ(I)> | 11.1 | |
Completeness [%] | 99.6 | |
Redundancy | 6.2 | |
CC(1/2) | 0.996 | 0.568 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 22% (w/v) PEG 3350 and 4% (v/v) Tacsimate (pH 6.0) |