8K8H
Crystal structure of the CysR-CTLD3 fragment of human DEC205
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-01-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 141.582, 79.205, 89.542 |
Unit cell angles | 90.00, 121.53, 90.00 |
Refinement procedure
Resolution | 29.640 - 2.790 |
R-factor | 0.1977 |
Rwork | 0.191 |
R-free | 0.26580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6inn |
RMSD bond length | 0.009 |
RMSD bond angle | 1.091 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.640 | 2.860 |
High resolution limit [Å] | 2.790 | 2.790 |
Rpim | 0.073 | 0.153 |
Number of reflections | 21098 | 2214 |
<I/σ(I)> | 13.3 | |
Completeness [%] | 99.4 | |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 293 | 0.2 M Sodium Citrate, 20% PEG3350, final pH 6.5 |