8K8G
De novo design protein -N9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 35.011, 75.997, 129.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.610 - 1.350 |
| R-factor | 0.2031 |
| Rwork | 0.202 |
| R-free | 0.22730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.842 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.210 | 1.370 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Number of reflections | 38537 | 1970 |
| <I/σ(I)> | 21.2 | |
| Completeness [%] | 100.0 | |
| Redundancy | 9 | |
| CC(1/2) | 1.000 | 0.817 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 289 | 20% w/v PEG 10000 0.1M Hepes ph7.5 |
