8K8A

Crystal structure of NFIL3 in complex with TTACGTAA DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 2
Unit cell lengths	74.030, 96.950, 38.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.090 - 2.070
R-factor	0.2297
Rwork	0.227
R-free	0.27610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.936
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.820	2.170
High resolution limit [Å]	2.060	2.060
Number of reflections	17871	2578
<I/σ(I)>	16.7
Completeness [%]	98.8
Redundancy	6.9
CC(1/2)	0.999	0.904

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	291	0.1 M Sodium acetate trihydrate pH 5.6, 8% w/v Polyethylene glycol 4000