8K86

Crystal structure of NFIL3 in complex with TTATGTAA DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 2
Unit cell lengths	74.084, 97.520, 39.007
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.910 - 2.060
R-factor	0.2267
Rwork	0.223
R-free	0.25930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.875
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.990	2.180
High resolution limit [Å]	2.060	2.060
Rmerge	0.091
Number of reflections	18067	2581
<I/σ(I)>	14.7
Completeness [%]	100.0
Redundancy	9.8
CC(1/2)	0.998	0.916

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	291	0.1 M Sodium acetate trihydrate pH 5.6, 8% w/v Polyethylene glycol 4000