8K7Y
Crystal structure of GH146 beta-L-arabinofuranosidase Bll3HypBA1 (amino acids 380-1051), ligand-free form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.231, 83.510, 102.034 |
| Unit cell angles | 90.00, 90.66, 90.00 |
Refinement procedure
| Resolution | 43.570 - 1.700 |
| R-factor | 0.2221 |
| Rwork | 0.220 |
| R-free | 0.25941 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.328 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.200 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.076 | 0.939 |
| Rpim | 0.065 | 0.787 |
| Number of reflections | 137533 | 6721 |
| <I/σ(I)> | 7.3 | 1 |
| Completeness [%] | 99.1 | 98.3 |
| Redundancy | 3.4 | 3.4 |
| CC(1/2) | 0.998 | 0.676 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.15 | 45% MPD, 0.2 M ammonium acetate, and 0.1 M HEPES-NaOH (pH 7.5) |
