8K7X
Crystal structure of GH146 beta-L-arabinofuranosidase Bll3HypBA1 (amino acids 380-1223) in complex with Tris
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-14 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.592, 111.571, 153.010 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.760 - 1.750 |
R-factor | 0.14414 |
Rwork | 0.143 |
R-free | 0.16730 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.640 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.760 | 1.780 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.176 | 1.014 |
Rpim | 0.102 | 0.592 |
Number of reflections | 103620 | 5038 |
<I/σ(I)> | 8.9 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.7 | 6.8 |
CC(1/2) | 0.993 | 0.696 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | 12% PEG 8000, 0.2 M MgCl2, and 0.1 M Tris-HCl (pH 8.5) |