8K7N
Structure of ferric-binding protein KfuA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.275, 52.278, 56.392 |
| Unit cell angles | 90.00, 94.43, 90.00 |
Refinement procedure
| Resolution | 37.580 - 2.000 |
| R-factor | 0.1625 |
| Rwork | 0.158 |
| R-free | 0.19880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.864 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.290 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rpim | 0.031 | 0.073 |
| Number of reflections | 18079 | 882 |
| <I/σ(I)> | 20.6 | |
| Completeness [%] | 96.7 | |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2 M Sodium chloride, 25% w/v Polyethylene glycol 3,350 and 0.1 M Tris |
