8K76

Crystal structure of S-adenosylmethionine-dependent methyltransferase from Fusobacterium nucleatum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	62.981, 80.629, 108.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.000 - 1.490
R-factor	0.2146
Rwork	0.214
R-free	0.22650
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.561
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.000	1.520
High resolution limit [Å]	1.489	1.490
Rmerge	0.097
Number of reflections	1036260	42568
<I/σ(I)>	11.8
Completeness [%]	99.9
Redundancy	5.9
CC(1/2)		0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	PEG 3350,sodium citrate, ammonium acetate