8K75
N-terminal domain of FIPV nucleocapsid protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-20 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97861 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.768, 60.900, 35.621 |
| Unit cell angles | 90.00, 97.92, 90.00 |
Refinement procedure
| Resolution | 30.550 - 1.160 |
| R-factor | 0.1684 |
| Rwork | 0.168 |
| R-free | 0.18383 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.820 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.180 |
| High resolution limit [Å] | 1.160 | 3.150 | 1.160 |
| Rmerge | 0.063 | 0.053 | 0.187 |
| Rmeas | 0.069 | 0.057 | 0.207 |
| Rpim | 0.026 | 0.022 | 0.088 |
| Total number of observations | 270478 | ||
| Number of reflections | 41565 | 2162 | 1972 |
| <I/σ(I)> | 7.1 | ||
| Completeness [%] | 99.0 | 99.8 | 94.8 |
| Redundancy | 6.5 | 6.7 | 5.2 |
| CC(1/2) | 0.991 | 0.993 | 0.978 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Potassium thiocyanate, 30% w/v Polyethylene glycol monomethyl ether 2,000 |
