8K70

Structural basis for the distinct roles of non-conserved Pro116 and conserved Tyr124 of BCH domain of yeast p50RhoGAP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 61
Unit cell lengths	108.110, 108.110, 250.803
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.810 - 2.810
R-factor	0.2125
Rwork	0.211
R-free	0.23950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.212
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.640	2.870
High resolution limit [Å]	2.800	2.800
Rmerge		0.230
Number of reflections	37961	2084
<I/σ(I)>	19.7
Completeness [%]	93.8
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	0.1 M Bis-Tris propane pH 7.0 and 2.1 M NaCl