8K6T
The minor pilin structure of FctB3 in Streptococcus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-01 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.100 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 44.335, 44.335, 181.092 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.060 - 2.800 |
R-factor | 0.2114 |
Rwork | 0.209 |
R-free | 0.25960 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.540 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.060 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 4687 | 2261 |
<I/σ(I)> | 10.7 | |
Completeness [%] | 96.2 | |
Redundancy | 3.2 | |
CC(1/2) | 0.995 | 0.927 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | ammonium sulfate, sodium acetate trihydrate |