8K6G
Crystal structure of E.coli Cyanase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-05-03 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 78.036, 81.110, 81.099 |
| Unit cell angles | 70.48, 76.30, 65.38 |
Refinement procedure
| Resolution | 48.950 - 1.500 |
| R-factor | 0.1707 |
| Rwork | 0.171 |
| R-free | 0.19020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.965 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.140 | 0.600 |
| Number of reflections | 237786 | 11829 |
| <I/σ(I)> | 17.24 | |
| Completeness [%] | 87.8 | 87.6 |
| Redundancy | 3.9 | 3.8 |
| CC(1/2) | 0.970 | 0.750 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7.3 | 293 | 50mM Tris-Cl (pH 7.3), 50mM potassium phosphate, and 2.5 M ammonium sulfate |
