8K5U

Se-glycosyltransferase RsSenB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	193.15
Detector technology	PIXEL
Collection date	2023-07-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 1 21 1
Unit cell lengths	80.826, 73.790, 81.280
Unit cell angles	90.00, 116.11, 90.00

Refinement procedure

Resolution	40.540 - 2.150
R-factor	0.2172
Rwork	0.216
R-free	0.25160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.906
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.540	2.220
High resolution limit [Å]	2.150	2.150
Number of reflections	46832	4046
<I/σ(I)>	9.6
Completeness [%]	99.9
Redundancy	6.6
CC(1/2)	0.995	0.802

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.2 M Ammonium citrate dibasic 20% PEG 3350