8K5Q
Crystal structure of YajQ STM0435 with c-di-GMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-01-02 |
Detector | DECTRIS EIGER2 S 9M |
Wavelength(s) | 0.987 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 26.984, 51.697, 126.267 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.570 - 2.280 |
R-factor | 0.2171 |
Rwork | 0.214 |
R-free | 0.27610 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.198 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 126.270 | 2.420 |
High resolution limit [Å] | 2.280 | 2.280 |
Rmerge | 0.108 | |
Rmeas | 0.113 | |
Rpim | 0.031 | |
Number of reflections | 8194 | 983 |
<I/σ(I)> | 17.4 | |
Completeness [%] | 95.1 | |
Redundancy | 12.3 | |
CC(1/2) | 0.997 | 0.966 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 20% PEG3350, 0.2M Lithium acetate dihydrate |