8K5L

Structure of tRNA (cmo5U34)-methyltransferase from Fusobacterium nucleatum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 1
Unit cell lengths	58.955, 58.980, 139.690
Unit cell angles	90.03, 90.03, 90.05

Refinement procedure

Resolution	31.067 - 1.670
R-factor	0.2265
Rwork	0.225
R-free	0.25670
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.797
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.070	1.710
High resolution limit [Å]	1.670	1.670
Rmeas		0.058
Number of reflections	204553	10475
<I/σ(I)>	1.38
Completeness [%]	93.8
Redundancy	3.3
CC(1/2)	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	287	28% peg 2000, 200mM ammonia sulfate, 0.1M Sodium acetate pH 4.6