8K5L
Structure of tRNA (cmo5U34)-methyltransferase from Fusobacterium nucleatum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-11-18 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.987 |
Spacegroup name | P 1 |
Unit cell lengths | 58.955, 58.980, 139.690 |
Unit cell angles | 90.03, 90.03, 90.05 |
Refinement procedure
Resolution | 31.067 - 1.670 |
R-factor | 0.2265 |
Rwork | 0.225 |
R-free | 0.25670 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.797 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.15.2_3472: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.070 | 1.710 |
High resolution limit [Å] | 1.670 | 1.670 |
Rmeas | 0.058 | |
Number of reflections | 204553 | 10475 |
<I/σ(I)> | 1.38 | |
Completeness [%] | 93.8 | |
Redundancy | 3.3 | |
CC(1/2) | 0.995 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 287 | 28% peg 2000, 200mM ammonia sulfate, 0.1M Sodium acetate pH 4.6 |