8K4W

Structure of a triple-helix region of human collagen type I from Trautec

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.00000
Spacegroup name	P 1
Unit cell lengths	24.837, 29.096, 85.236
Unit cell angles	95.53, 90.00, 90.00

Refinement procedure

Resolution	28.280 - 1.500
Rwork	0.193
R-free	0.26450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.473
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.280	1.530
High resolution limit [Å]	1.500	1.500
Number of reflections	36638	1834
<I/σ(I)>	10.9
Completeness [%]	96.2
Redundancy	2.9
CC(1/2)	0.997	0.881

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M NaCl, 0.1M Bis-Tris pH5.5, 25% PEG3,350