8K4L

Crystal structure of NRF1 homodimer in complex with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-02
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	37.757, 118.827, 121.821
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.430 - 2.100
R-factor	0.1957
Rwork	0.193
R-free	0.24310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.061
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.19_4092))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.530	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.072	0.568
Number of reflections	32991	2615
<I/σ(I)>	18.2	4.4
Completeness [%]	100.0
Redundancy	12.3
CC(1/2)	0.978	0.955

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	2% (v/v) Tacsimate (pH 6.0), 0.1 M Bis-Tris (pH 6.5),20% (w/v) Polyethylene glycol 3350