8K3F

Crystal structure of the recombination mediator protein RecR from Campylobacter jejuni

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2021-06-30
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	C 1 2 1
Unit cell lengths	231.427, 64.186, 74.621
Unit cell angles	90.00, 100.60, 90.00

Refinement procedure

Resolution	29.178 - 2.603
R-factor	0.2086
Rwork	0.207
R-free	0.24620
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.630
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.640
High resolution limit [Å]	2.600	2.600
Number of reflections	32226	1614
<I/σ(I)>	19.5	2.1
Completeness [%]	97.0	97.2
Redundancy	2.8	2.8
CC(1/2)	0.992	0.666

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	ethanol, Hepes, magnesium chloride