8K3F
Crystal structure of the recombination mediator protein RecR from Campylobacter jejuni
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-06-30 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9793 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 231.427, 64.186, 74.621 |
Unit cell angles | 90.00, 100.60, 90.00 |
Refinement procedure
Resolution | 29.178 - 2.603 |
R-factor | 0.2086 |
Rwork | 0.207 |
R-free | 0.24620 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.630 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.15.2_3472: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 32226 | 1614 |
<I/σ(I)> | 19.5 | 2.1 |
Completeness [%] | 97.0 | 97.2 |
Redundancy | 2.8 | 2.8 |
CC(1/2) | 0.992 | 0.666 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | ethanol, Hepes, magnesium chloride |