8K3D

Crystal structure of NRF1 DBD bound to DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-X
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-14
Detector	RIGAKU HyPix-3000
Wavelength(s)	1.54184
Spacegroup name	C 2 2 21
Unit cell lengths	63.843, 64.706, 98.522
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.050 - 2.300
R-factor	0.1963
Rwork	0.194
R-free	0.23900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.015
Data reduction software	CrysalisPro (CrysAlisPro 1.171.42.36a)
Data scaling software	CrysalisPro
Phasing software	PHASER
Refinement software	PHENIX ((1.19_4092: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.050	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.080	0.331
Number of reflections	9050	889
<I/σ(I)>	7	1.6
Completeness [%]	97.3
Redundancy	2.9
CC(1/2)	0.993	0.874

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M Ammonium sulfate, 0.1 M Bis-Tris (pH 6.5), and 25% (w/v) Polyethylene glycol 3350