8K2Z

Crystal structure of a human mascRNA A2G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97852
Spacegroup name	P 1 21 1
Unit cell lengths	79.365, 30.111, 89.353
Unit cell angles	90.00, 105.73, 90.00

Refinement procedure

Resolution	28.670 - 2.400
R-factor	0.2775
Rwork	0.277
R-free	0.29220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.149
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.760	2.460
High resolution limit [Å]	2.400	2.400
Rmerge	0.095	0.783
Number of reflections	16486	1215
<I/σ(I)>	7.8	1.7
Completeness [%]	99.9	100
Redundancy	6.2
CC(1/2)	0.995	0.497

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	0.08 M Strontium chloride hexahydrate, 0.02 M Magnesium chloride hexahydrate, 18% v/v (+ / -)-2-Methyl-2,4-pentanediol, 0.012 M Spermine tetrahydrochloride