8K2Y
Crystal structure of MucD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 193.15 |
| Detector technology | PIXEL |
| Collection date | 2023-03-04 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 133.703, 91.461, 81.033 |
| Unit cell angles | 90.00, 116.06, 90.00 |
Refinement procedure
| Resolution | 29.560 - 2.700 |
| R-factor | 0.2105 |
| Rwork | 0.205 |
| R-free | 0.26880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AlphaFold |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.347 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.560 | 2.900 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.862 | 0.862 |
| Number of reflections | 24057 | 24042 |
| <I/σ(I)> | 11.68 | |
| Completeness [%] | 99.3 | |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 2.0 M (NH4)2SO4, 0.1 M cacodylate pH 6.5, 0.2 M NaCl |
