8K2N
Crystal structure of Group 4 Monosaccharide-releasing beta-N-acetylgalactosaminidase NgaLy from Lacticaseibacillus yichunensis, apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-23 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 82.930, 94.620, 116.180 |
| Unit cell angles | 70.93, 73.81, 72.43 |
Refinement procedure
| Resolution | 48.790 - 2.500 |
| R-factor | 0.18556 |
| Rwork | 0.183 |
| R-free | 0.24147 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.107 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.010 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 107859 | 5311 |
| <I/σ(I)> | 7.1 | |
| Completeness [%] | 100.0 | |
| Redundancy | 2 | |
| CC(1/2) | 0.963 | 0.672 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1 M sodium acetate pH 4.2, 25% w/v Polyethylene glycol 3,350 |
