8K1R
YeeE(TsuA)-YeeD(TsuB) complex for thiosulfate uptake
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-06-08 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 73.580, 101.750, 182.760 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.930 - 2.600 |
R-factor | 0.208 |
Rwork | 0.206 |
R-free | 0.25340 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.161 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.930 | 2.693 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.988 | 5.227 |
Rmeas | 0.989 | 5.236 |
Rpim | 0.048 | 0.271 |
Number of reflections | 42977 | 4206 |
<I/σ(I)> | 15.25 | 2.1 |
Completeness [%] | 99.5 | 99.32 |
Redundancy | 321.2 | 297.1 |
CC(1/2) | 0.996 | 0.861 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | 0.35 M Ammonium formate, 0.1M Tris-HCl pH 8.0-9.0, 26-36% 1,4- Butandiol |