8K1F
Crystal Structure of TrmR from Fusobacterium nucleatum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2022-06-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 165.281, 46.589, 87.316 |
Unit cell angles | 90.00, 98.33, 90.00 |
Refinement procedure
Resolution | 36.097 - 2.400 |
R-factor | 0.2206 |
Rwork | 0.213 |
R-free | 0.29230 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 0.947 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.15.2_3472: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.100 | 2.486 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 110870 | 23951 |
<I/σ(I)> | 11.2 | |
Completeness [%] | 80.8 | |
Redundancy | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 295 | 0.1M MES, 9% PEG 8000, 95mM calcium acetate |