8K0Y

Crystal structure of a medaka mascRNA U23G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	159.360, 29.250, 98.080
Unit cell angles	90.00, 118.56, 90.00

Refinement procedure

Resolution	28.080 - 2.280
Rwork	0.249
R-free	0.28540
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.961
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.720	2.340
High resolution limit [Å]	2.280	2.280
Rmerge	0.079	1.174
Number of reflections	17707	1336
<I/σ(I)>	12.4	1.6
Completeness [%]	99.6	99.8
Redundancy	6.1	5.5
CC(1/2)	0.997	0.618

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	291	0.08 M Strontium chloride hexahydrate, 0.02 M Magnesium chloride hexahydrate, 18% v/v (+ / -)-2-Methyl-2,4-pentanediol, 0.012 M Spermine tetrahydrochloride