8K0J

Crystal structure of Cas7f

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 61
Unit cell lengths	100.516, 100.516, 54.669
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.999 - 2.365
R-factor	0.194
Rwork	0.193
R-free	0.22190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.503
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.450
High resolution limit [Å]	2.365	2.365
Rmerge	0.146	0.155
Number of reflections	12986	1284
<I/σ(I)>	32.9
Completeness [%]	99.7
Redundancy	14.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 400, Sodium chloride