8K0H
Structure of Cas5f-8f
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.575, 68.186, 73.631 |
| Unit cell angles | 90.00, 109.72, 90.00 |
Refinement procedure
| Resolution | 46.692 - 2.560 |
| R-factor | 0.1941 |
| Rwork | 0.192 |
| R-free | 0.23380 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.707 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.650 |
| High resolution limit [Å] | 2.560 | 2.560 |
| Rmerge | 0.141 | 0.645 |
| Number of reflections | 14740 | 1351 |
| <I/σ(I)> | 18.3 | |
| Completeness [%] | 98.5 | |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 3350, KCl |
