8K05

Pseudouridine 5'-monophosphate glycosylase from Arabidopsis thaliana -- sulfate bound holoenzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2019-04-09
Detector	ADSC QUANTUM 270
Wavelength(s)	0.979
Spacegroup name	P 21 3
Unit cell lengths	121.126, 121.126, 121.126
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.550 - 1.450
R-factor	0.2058
Rwork	0.206
R-free	0.21710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.059
Data scaling software	HKL-2000
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.500
High resolution limit [Å]	1.450	3.120	1.450
Rmerge	0.111	0.054	3.706
Rmeas	0.113	0.055	3.794
Rpim	0.024	0.012	0.810
Total number of observations	2288355
Number of reflections	104528	10687	10315
<I/σ(I)>	6
Completeness [%]	99.9	99.2	100
Redundancy	21.9	20.6	21.9
CC(1/2)	0.999	0.999	0.455

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	0.1M Cacodylate pH6.5, 1.4M Ammonium sulfate