8K00

RPA70N-MRE11 fusion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97854
Spacegroup name	P 21 21 21
Unit cell lengths	38.900, 53.705, 55.329
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.380 - 1.400
R-factor	0.1744
Rwork	0.173
R-free	0.19880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7xv4
RMSD bond length	0.004
RMSD bond angle	0.745
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.21rc1_4924: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.380	1.430
High resolution limit [Å]	1.400	1.400
Rmerge	0.058	0.081
Number of reflections	44033	2576
<I/σ(I)>	23.61
Completeness [%]	99.8
Redundancy	6.3
CC(1/2)	0.996	0.994

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	Ammonium sulate