8JZW
Cystal structure of HP1 in complex with TRIM66 peptide
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 293.15 |
| Detector technology | PIXEL |
| Collection date | 2017-04-20 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.247, 98.690, 40.741 |
| Unit cell angles | 90.00, 97.46, 90.00 |
Refinement procedure
| Resolution | 35.940 - 1.800 |
| R-factor | 0.1916 |
| Rwork | 0.190 |
| R-free | 0.22480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.905 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.091 | 0.576 |
| Number of reflections | 25428 | 2491 |
| <I/σ(I)> | 6.9 | 2.188 |
| Completeness [%] | 96.5 | 94.9 |
| Redundancy | 6.9 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 293.15 | 30% PEG MME 2000, 0.1 mmol/L sodium cacodylate (pH 6.0) |
